› 4xe9

X-ray diffraction
1.84Å resolution

Crystal structure of the NanB sialidase from Streptococcus pneumoniae in complex with Optactin

Released:
DOI: 10.2210/pdb4xe9/pdb
PDB entry 4xe9 coloured by chain, front view.
PDB entry 4xe9 coloured by chain, side view.
PDB entry 4xe9 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry author: Brear P

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase B Chain: A
Molecule details ›
Chain: A
Length: 658 amino acids
Theoretical weight: 73.52 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q54727 (Residues: 39-696; Coverage: 99%)
Gene names: SP_1687, nanB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand OPX 1 x OPX
Ligand DMS 5 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 76.201Å b: 82.702Å c: 116.423Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.207
Expression system: Escherichia coli BL21(DE3)

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