› 4xd0

X-ray diffraction
1.8Å resolution

X-ray structure of the N-formyltransferase QdtF from Providencia alcalifaciens

Released:
DOI: 10.2210/pdb4xd0/pdb
PDB entry 4xd0 coloured by chain, front view.
PDB entry 4xd0 coloured by chain, side view.
PDB entry 4xd0 coloured by chain, top view.
Source organism: Providencia alcalifaciens
Primary publication:
New role for the ankyrin repeat revealed by a study of the N-formyltransferase from Providencia alcalifaciens.
Woodford CR, Thoden JB, Holden HM
Biochemistry 54 631-8 (2015)
PMID: 25574689

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyl transferase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 48.16 KDa
Source organism: Providencia alcalifaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: F8RC03 (Residues: 1-397; Coverage: 100%)
Gene name: qdtf
Sequence domains:

Ligands and Environments


Cofactor: Ligand FON 1 x FON
4 bound ligands:
Ligand T3F 2 x T3F
Ligand K 1 x K
Ligand NA 3 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 78.134Å b: 78.134Å c: 151.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.265
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Enzymes required for the biosynthesis of N-formylated sugars.
Holden et al. (2016)
15 other articles

1 mention without citation

New role for the ankyrin repeat revealed by a study of the N-formyltransferase from Providencia alcalifaciens.
Woodford et al. (2015)