› 4xcx

X-ray diffraction
2.84Å resolution

METHYLTRANSFERASE DOMAIN OF SMALL RNA 2'-O-METHYLTRANSFERASE

Released:
DOI: 10.2210/pdb4xcx/pdb
PDB 4xcx coloured by chain and viewed from the front.
PDB 4xcx coloured by chain and viewed from the side.
PDB 4xcx coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Walker JR, Zeng H, Dong A, Li Y, Wernimont A, Bountra C, Arrowsmith CH, Edwards AM, Brown PJ, Wu H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + small RNA = S-adenosyl-L-homocysteine + small RNA containing a 3'-terminal 2'-O-methylnucleotide
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small RNA 2'-O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5T8I9 (Residues: 14-262; Coverage: 63%)
Gene names: C1orf59, HENMT1
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 166.025Å b: 166.025Å c: 74.478Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.225 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links