4xcv

X-ray diffraction
1.4Å resolution

Probable 2-hydroxyacid dehydrogenase from Rhizobium etli CFN 42 in complex with NADPH

Released:
Source organism: Rhizobium etli CFN 42
Entry authors: Langner KM, Shabalin IG, Handing KB, Gasiorowska OA, Stead M, Hillerich BS, Chowdhury S, Hammonds J, Zimmerman MD, Al Obadi N, Bonanno J, Seidel R, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-Hacid_dh_C domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 35.52 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2KDT2 (Residues: 1-319; Coverage: 100%)
Gene name: RHE_CH00179
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 65.663Å b: 65.663Å c: 151.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.178
Expression system: Escherichia coli BL21(DE3)