› 4xcn

X-ray diffraction
2.9Å resolution

Crystal structure of human 4E10 Fab in complex with phosphatidic acid (06:0 PA); 2.9 A resolution

Released:
DOI: 10.2210/pdb4xcn/pdb
PDB entry 4xcn coloured by chain, front view.
PDB entry 4xcn coloured by chain, side view.
PDB entry 4xcn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Irimia A, Sarkar A, Stanfield RL, Wilson IA
Immunity 44 21-31 (2016)
PMID: 26777395

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230427 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
4E10 Fab light chain Chains: B, D, F, I, K, L
Molecule details ›
Chains: B, D, F, I, K, L
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
4E10 Fab heavy chain Chains: A, C, E, G, H, J
Molecule details ›
Chains: A, C, E, G, H, J
Length: 230 amino acids
Theoretical weight: 24.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand UNL 6 x UNL
Ligand 44E 2 x 44E
Ligand GOL 1 x GOL
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6122
Unit cell:
a: 149.89Å b: 149.89Å c: 469.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.246
Expression system: Homo sapiens

Quick links

Citations

14 review citations

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
13 more

3 mentions without citation

Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Irimia et al. (2016)
2 more