› 4xcc

X-ray diffraction
2.81Å resolution

Crystal structure of human 4E10 Fab crystalized in the presence of Phosphatidylcholine (06:0 PC); I422 space group

Released:
DOI: 10.2210/pdb4xcc/pdb
PDB entry 4xcc coloured by chain, front view.
PDB entry 4xcc coloured by chain, side view.
PDB entry 4xcc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Irimia A, Sarkar A, Stanfield RL, Wilson IA
Immunity 44 21-31 (2016)
PMID: 26777395

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230425 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
4E10 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
4E10 Fab Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I422
Unit cell:
a: 108.071Å b: 108.071Å c: 186.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.272
Expression system: Homo sapiens

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Citations

14 review citations

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
13 more

1 mention without citation

Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Irimia et al. (2016)