4xc9

X-ray diffraction
2.4Å resolution

Crystal Structure of apo HygX from Streptomyces hygroscopicus

Released:
Entry authors: McCulloch KM, McCranie EK, Sarwar M, Mathieu JL, Gitschlag BL, Du Y, Bachmann BO, Iverson TM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative oxidase/hydroxylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 258 amino acids
Theoretical weight: 28.98 KDa
Source organism: Streptomyces hygroscopicus subsp. hygroscopicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2MFS1 (Residues: 2-258; Coverage: 100%)
Gene name: hygX
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)
Structure domains: q2cbj1_9rhob like domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 112.113Å b: 274.427Å c: 52.825Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.246
Expression system: Escherichia coli BL21(DE3)