› 4xab

X-ray diffraction
1.75Å resolution

Crystal Structure of EvdO2 from Micromonospora carbonacea var. aurantiaca

Released:
DOI: 10.2210/pdb4xab/pdb
PDB entry 4xab coloured by chain, front view.
PDB entry 4xab coloured by chain, side view.
PDB entry 4xab coloured by chain, top view.
Source organism: Micromonospora carbonacea
Primary publication:
Oxidative cyclizations in orthosomycin biosynthesis expand the known chemistry of an oxygenase superfamily.
McCulloch KM, McCranie EK, Smith JA, Sarwar M, Mathieu JL, Gitschlag BL, Du Y, Bachmann BO, Iverson TM
Proc Natl Acad Sci U S A 112 11547-52 (2015)
PMID: 26240321

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EvdO2 Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 28.61 KDa
Source organism: Micromonospora carbonacea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KL01 (Residues: 1-252; Coverage: 100%)
Gene names: HXZ27_04030, evdO2
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)
Structure domains: q2cbj1_9rhob like domain

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 42.612Å b: 65.803Å c: 84.462Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Oxidative Cyclization in Natural Product Biosynthesis.
Tang et al. (2017)
5 more

1 mention without citation

Oxidative cyclizations in orthosomycin biosynthesis expand the known chemistry of an oxygenase superfamily.
McCulloch et al. (2015)