X-ray diffraction
1.9Å resolution

Crystal structure of D-isomer specific 2-hydroxyacid dehydrogenase from Xanthobacter autotrophicus Py2

Entry authors: Handing KB, Gasiorowska OA, Shabalin IG, Cymborowski MT, Bonanno J, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2-Hacid_dh_C domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 34.71 KDa
Source organism: Xanthobacter autotrophicus Py2
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A7IIH0 (Residues: 1-319; Coverage: 100%)
Gene name: Xaut_2571
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2221
Unit cell:
a: 58.762Å b: 63.714Å c: 154.967Å
α: 90° β: 90° γ: 90°
R R work R free
0.166 0.163 0.209
Expression system: Escherichia coli BL21(DE3)