› 4x9x

X-ray diffraction
1.2Å resolution

Biochemical Roles for Conserved Residues in the Bacterial Fatty Acid Binding Protein Family

Released:
DOI: 10.2210/pdb4x9x/pdb
PDB entry 4x9x coloured by chain, front view.
PDB entry 4x9x coloured by chain, side view.
PDB entry 4x9x coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus MW2
Primary publication:
Biochemical Roles for Conserved Residues in the Bacterial Fatty Acid-binding Protein Family.
Broussard TC, Miller DJ, Jackson P, Nourse A, White SW, Rock CO
J Biol Chem 291 6292-303 (2016)
PMID: 26774272

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DegV domain-containing protein MW1315 Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.85 KDa
Source organism: Staphylococcus aureus subsp. aureus MW2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NWR0 (Residues: 1-279; Coverage: 100%)
Gene name: MW1315
Sequence domains: Uncharacterised protein, DegV family COG1307
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand OLA 1 x OLA
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 167.343Å b: 41.872Å c: 38.573Å
α: 90° β: 94.49° γ: 90°
R-values:
R R work R free
0.119 0.118 0.144
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Exogenous fatty acid metabolism in bacteria.
Yao et al. (2017)
1 more

4 mentions without citation

A fatty acid-binding protein of Streptococcus pneumoniae facilitates the acquisition of host polyunsaturated fatty acids.
Gullett et al. (2019)
3 more