› 4x9f

X-ray diffraction
2.35Å resolution

Crystal structure of Dscam1 isoform 6.9, N-terminal four Ig domains

Released:
DOI: 10.2210/pdb4x9f/pdb
PDB 4x9f coloured by chain and viewed from the front.
PDB 4x9f coloured by chain and viewed from the side.
PDB 4x9f coloured by chain and viewed from the top.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis of Dscam1 homodimerization: Insights into context constraint for protein recognition.
Li SA, Cheng L, Yu Y, Wang JH, Chen Q
Sci Adv 2 e1501118 (2016)
PMID: 27386517

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Down syndrome cell adhesion molecule 1, isoform BE Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 43.55 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q0E9K4 (Residues: 34-425; Coverage: 20%)
Gene names: 43Bc, CG17800, CT39257, DSCAM, DScam, DmDscam, Dm_2R:13579, Dm_2R:13612, Dmel\CG17800, Dmel_CG17800, DsCam, Dscam, Dscam-hv, Dscam1, Dsm, Neu1, dScam, dscam, dscam1, l(2)05518, l(2)43Bc, p270
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand GOL 4 x GOL
Ligand SO4 15 x SO4
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 63.686Å b: 90.5Å c: 169.601Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.26
Expression system: Trichoplusia ni

Quick links

Citations

2 review citations

Glycosylation in Axonal Guidance.
Mutalik et al. (2021)
1 more