› 4x8r

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM Rhodobacter sphaeroides (Rsph17029_2138, TARGET EFI-510205) WITH BOUND Glucuronate

Released:
DOI: 10.2210/pdb4x8r/pdb
PDB entry 4x8r coloured by chain, front view.
PDB entry 4x8r coloured by chain, side view.
PDB entry 4x8r coloured by chain, top view.
Source organism: Cereibacter sphaeroides ATCC 17029
Entry authors: Yadava U, Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP dicarboxylate transporter, DctP subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 37.45 KDa
Source organism: Cereibacter sphaeroides ATCC 17029
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3PLM5 (Residues: 22-325; Coverage: 100%)
Gene name: Rsph17029_2138
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

2 bound ligands:
Ligand BDP 2 x BDP
Ligand PO4 4 x PO4
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P42212
Unit cell:
a: 116.8Å b: 116.8Å c: 111.937Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.201
Expression system: Escherichia coli BL21(DE3)

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