4x81

X-ray diffraction
1.59Å resolution

MycF mycinamicin III 3'-O-methyltransferase (E35Q, M56A, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)

Released:
Source organism: Micromonospora griseorubida
Entry authors: Bernard SM, Smith JL

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + mycinamicin III = S-adenosyl-L-homocysteine + mycinamicin IV
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycinamicin III 3''-O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 30.7 KDa
Source organism: Micromonospora griseorubida
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q49492 (Residues: 1-254; Coverage: 100%)
Gene name: mycF
Sequence domains: Macrocin-O-methyltransferase (TylF)

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 50.269Å b: 91.747Å c: 127.774Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.188
Expression system: Escherichia coli BL21