X-ray diffraction
2.15Å resolution

Crystal structure of S. aureus TarM G117R mutant in complex with Fondaparinux, alpha-GlcNAc-glycerol and UDP


Function and Biology Details

Reaction catalysed:
n UDP-N-acetyl-alpha-D-glucosamine + 4-O-(D-ribitylphospho)(n)-di((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n UDP + 4-O-(2-N-acetyl-alpha-D-glucosaminyl-D-ribitylphospho)(n)-di((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Poly(ribitol-phosphate) alpha-N-acetylglucosaminyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 57.46 KDa
Source organism: Staphylococcus aureus subsp. aureus 21178
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0A0H2WWV6 (Residues: 1-493; Coverage: 100%)
Gene names: SACOL1043, tarM
Sequence domains: Glycosyl transferases group 1
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

Carbohydrate polymer : NEW Components: ZDO, IDS, SUS, BDP, SGN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P1
Unit cell:
a: 43.47Å b: 92.14Å c: 96.11Å
α: 65.89° β: 83.66° γ: 84.12°
R R work R free
0.18 0.179 0.2
Expression system: Escherichia coli BL21(DE3)