X-ray diffraction
3.19Å resolution

Crystal structure of S. aureus TarM in complex with UDP


Function and Biology Details

Reaction catalysed:
n UDP-N-acetyl-alpha-D-glucosamine + 4-O-(D-ribitylphospho)(n)-di((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n UDP + 4-O-(2-N-acetyl-alpha-D-glucosaminyl-D-ribitylphospho)(n)-di((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Poly(ribitol-phosphate) alpha-N-acetylglucosaminyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 493 amino acids
Theoretical weight: 57.36 KDa
Source organism: Staphylococcus aureus subsp. aureus 21178
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0A0H2WWV6 (Residues: 1-493; Coverage: 100%)
Gene names: SACOL1043, tarM
Sequence domains: Glycosyl transferases group 1
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P63
Unit cell:
a: 162.289Å b: 162.289Å c: 228.024Å
α: 90° β: 90° γ: 120°
R R work R free
0.167 0.165 0.191
Expression system: Escherichia coli BL21(DE3)