› 4x5w

X-ray diffraction
1.34Å resolution

HLA-DR1 with CLIP102-120(M107W)

Released:
DOI: 10.2210/pdb4x5w/pdb
PDB entry 4x5w coloured by chain, front view.
PDB entry 4x5w coloured by chain, side view.
PDB entry 4x5w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
MHC class II complexes sample intermediate states along the peptide exchange pathway.
Wieczorek M, Sticht J, Stolzenberg S, Günther S, Wehmeyer C, El Habre Z, Álvaro-Benito M, Noé F, Freund C
Nat Commun 7 13224 (2016)
PMID: 27827392

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135318 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 22.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01903 (Residues: 26-217; Coverage: 84%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 199 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01911 (Residues: 30-227; Coverage: 84%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Class-II-associated invariant chain peptide Chain: C
Molecule details ›
Chain: C
Length: 19 amino acids
Theoretical weight: 2.2 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04233 (Residues: 102-120; Coverage: 6%)
Gene names: CD74, DHLAG

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 73.775Å b: 90.851Å c: 138.793Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.17
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Peptide and Peptide-Dependent Motions in MHC Proteins: Immunological Implications and Biophysical Underpinnings.
Ayres et al. (2017)
7 more

7 mentions without citation

Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation.
Wieczorek et al. (2017)
6 more