X-ray diffraction
2.6Å resolution

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a human MenBeta minihelix ending in CCACC and AMPcPP


Function and Biology Details

Reaction catalysed:
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CCA-adding enzyme Chains: A, C
Molecule details ›
Chains: A, C
Length: 457 amino acids
Theoretical weight: 53.67 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
Structure domains:
Human MenBeta minihelix ending in CCACC Chains: B, D
Molecule details ›
Chains: B, D
Length: 37 nucleotides
Theoretical weight: 11.89 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 111.059Å b: 215.602Å c: 58.629Å
α: 90° β: 90° γ: 90°
R R work R free
0.187 0.184 0.237
Expression systems:
  • Escherichia coli
  • Not provided