4x4s

X-ray diffraction
3.25Å resolution

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACC and CTP

Released:

Function and Biology Details

Reaction catalysed:
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CCA-adding enzyme Chains: A, C
Molecule details ›
Chains: A, C
Length: 457 amino acids
Theoretical weight: 53.67 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
Structure domains:
G70A tRNA minihelix ending in CCACC Chains: B, D
Molecule details ›
Chains: B, D
Length: 37 nucleotides
Theoretical weight: 11.87 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 111.343Å b: 216.45Å c: 58.447Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.276
Expression systems:
  • Escherichia coli
  • Not provided