› 4x3l

X-ray diffraction
1.7Å resolution

Crystal Structure of TTHA0275 from Thermus thermophilus (HB8) in complex with 5'-methylthioadenosine in space group P21212

Released:
DOI: 10.2210/pdb4x3l/pdb
PDB 4x3l coloured by chain and viewed from the front.
PDB 4x3l coloured by chain and viewed from the side.
PDB 4x3l coloured by chain and viewed from the top.
Source organism: Thermus thermophilus HB8
Entry authors: Demirci H, Belardinelli R, Jogl G

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA 2-O ribose methyltransferase substrate binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 28.04 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLL8 (Residues: 1-260; Coverage: 100%)
Gene name: TTHA0275
Sequence domains: SpoU rRNA Methylase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MTA 2 x MTA
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 111.17Å b: 115.95Å c: 58.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.207
Expression system: Escherichia coli BL21(DE3)

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