4x28

X-ray diffraction
1.99Å resolution

Crystal structure of the ChsE4-ChsE5 complex from Mycobacterium tuberculosis

Released:
Entry authors: Guja KE, Yang M, Sampson N, Garcia-Diaz M

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acyl-CoA dehydrogenase FadE26 Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 44.3 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: I6YCA3 (Residues: 1-400; Coverage: 100%)
Gene names: LH57_19105, Rv3504, fadE26
Sequence domains:
Structure domains:
Acyl-CoA dehydrogenase FadE27 Chains: C, D
Molecule details ›
Chains: C, D
Length: 373 amino acids
Theoretical weight: 39.19 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: I6Y3Q0 (Residues: 1-373; Coverage: 100%)
Gene names: LH57_19110, Rv3505, fadE27
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 185.745Å b: 108.095Å c: 82.019Å
α: 90° β: 93.04° γ: 90°
R-values:
R R work R free
0.153 0.151 0.186
Expression system: Escherichia coli