› 4x1t

X-ray diffraction
2.25Å resolution

The crystal structure of Arabidopsis thaliana galactolipid synthase MGD1 in complex with UDP

Released:
DOI: 10.2210/pdb4x1t/pdb
PDB 4x1t coloured by chain and viewed from the front.
PDB 4x1t coloured by chain and viewed from the side.
PDB 4x1t coloured by chain and viewed from the top.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights and membrane binding properties of MGD1, the major galactolipid synthase in plants.
Rocha J, Sarkis J, Thomas A, Pitou L, Radzimanowski J, Audry M, Chazalet V, de Sanctis D, Palcic MM, Block MA, Girard-Egrot A, Mar��chal E, Breton C
Plant J. 85 622-33 (2016)
PMID: 26935252

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monogalactosyldiacylglycerol synthase 1, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 45.33 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O81770 (Residues: 137-533; Coverage: 75%)
Gene names: At4g31780, EMB2797, F28M20.30, MGD1, MGDA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand UDP 1 x UDP
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P63
Unit cell:
a: 139.25Å b: 139.25Å c: 38.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Cellular Organization and Regulation of Plant Glycerolipid Metabolism.
Lavell et al. (2019)
3 more