X-ray diffraction
2.1Å resolution

The crystal structure of mupain-1-12 in complex with murinised human uPA at pH7.4


Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mupain-1-12 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.11 KDa
Source organism: synthetic construct
Expression system: Not provided
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 247 amino acids
Theoretical weight: 27.87 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
  • Canonical: P00749 (Residues: 179-425; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: R3
Unit cell:
a: 121.177Å b: 121.177Å c: 42.368Å
α: 90° β: 90° γ: 120°
R R work R free
0.213 0.21 0.273
Expression systems:
  • Not provided
  • Komagataella pastoris