› 4x1j

X-ray diffraction
2.5Å resolution

X-ray crystal structure of the dimeric BMP antagonist NBL1

Released:
DOI: 10.2210/pdb4x1j/pdb
PDB entry 4x1j coloured by chain, front view.
PDB entry 4x1j coloured by chain, side view.
PDB entry 4x1j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of neuroblastoma suppressor of tumorigenicity 1 (NBL1): insights for the functional variability across bone morphogenetic protein (BMP) antagonists.
Nolan K, Kattamuri C, Luedeke DM, Angerman EB, Rankin SA, Stevens ML, Zorn AM, Thompson TB
J Biol Chem 290 4759-4771 (2015)
PMID: 25561725

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuroblastoma suppressor of tumorigenicity 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 12.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41271 (Residues: 17-132; Coverage: 70%)
Gene names: DAN, DAND1, NBL1
Sequence domains: DAN domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 59.665Å b: 59.665Å c: 145.018Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.216 0.273
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural perspective of BMP ligands and signaling.
Gipson et al. (2020)
16 other articles

6 mentions without citation

Structural Biology and Evolution of the TGF-β Family.
Hinck et al. (2016)
5 more