› 4x1e

X-ray diffraction
2.4Å resolution

Crystal structure of unliganded E. coli transcriptional regulator RutR, W167A mutant

Released:
DOI: 10.2210/pdb4x1e/pdb
PDB entry 4x1e coloured by chain, front view.
PDB entry 4x1e coloured by chain, side view.
PDB entry 4x1e coloured by chain, top view.
Source organism: Escherichia coli CFT073
Primary publication:
Ligand binding specificity of RutR, a member of the TetR family of transcription regulators in Escherichia coli.
Nguyen Le Minh P, de Cima S, Bervoets I, Maes D, Rubio V, Charlier D
FEBS Open Bio 5 76-84 (2015)
PMID: 25685666

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator RutR Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 24.6 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACU3 (Residues: 1-212; Coverage: 100%)
Gene names: c1150, rutR
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 48.42Å b: 91.77Å c: 97.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.
Bervoets et al. (2019)
1 more

1 mention without citation

Ligand binding specificity of RutR, a member of the TetR family of transcription regulators in Escherichia coli.
Nguyen Le Minh et al. (2015)