4x0b

X-ray diffraction
3.2Å resolution

Structure of tRNA-processing enzyme complex 7

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
A-adding tRNA nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 46.55 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66728 (Residues: 443-824; Coverage: 46%)
Gene name: aq_411
Sequence domains: Poly A polymerase head domain
Structure domains:
RNA (77-MER) Chain: B
Molecule details ›
Chain: B
Length: 77 nucleotides
Theoretical weight: 24.81 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21212
Unit cell:
a: 58.74Å b: 110.07Å c: 127.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.267 0.304
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided