4wym

X-ray diffraction
2.6Å resolution

Structural basis of HIV-1 capsid recognition by CPSF6

Released:
Entry authors: Battacharya A, Taylor AB, Hart PJ, Ivanov DN

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dodecamer
hetero undecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Capsid protein p24 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 231 amino acids
Theoretical weight: 25.46 KDa
Source organism: Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12493 (Residues: 133-363; Coverage: 46%)
Gene name: gag
Sequence domains:
Structure domains:
Cleavage and polyadenylation specificity factor subunit 6 Chains: M, N, O, P, Q, R, S, T, U, V, W
Molecule details ›
Chains: M, N, O, P, Q, R, S, T, U, V, W
Length: 17 amino acids
Theoretical weight: 1.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16630 (Residues: 276-290; Coverage: 3%)
Gene names: CFIM68, CPSF6

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 134.79Å b: 136.03Å c: 207.257Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli