4wyi

X-ray diffraction
2.5Å resolution

The crystal structure of Arabidopsis thaliana galactolipid synthase, MGD1 (apo-form)

Released:
Source organism: Arabidopsis thaliana
Entry authors: Rocha J, Breton C

Function and Biology Details

Reaction catalysed:
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monogalactosyldiacylglycerol synthase 1, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 45.33 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O81770 (Residues: 137-533; Coverage: 75%)
Gene names: At4g31780, EMB2797, F28M20.30, MGD1, MGDA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 59.61Å b: 65.619Å c: 80.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.2
Expression system: Escherichia coli BL21(DE3)