› 4wy7

X-ray diffraction
2.1Å resolution

Crystal structure of recombinant 4E10 expressed in Escherichia coli with epitope bound

Released:
DOI: 10.2210/pdb4wy7/pdb
PDB entry 4wy7 coloured by chain, front view.
PDB entry 4wy7 coloured by chain, side view.
PDB entry 4wy7 coloured by chain, top view.
Source organisms:
Primary publication:
The Atomic Structure of the HIV-1 gp41 Transmembrane Domain and Its Connection to the Immunogenic Membrane-proximal External Region.
Apellániz B, Rujas E, Serrano S, Morante K, Tsumoto K, Caaveiro JM, Jiménez MÁ, Nieva JL
J Biol Chem 290 12999-3015 (2015)
PMID: 25787074

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209272 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 4E10 Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 23.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Fab 4E10Light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 2.19 KDa
Source organism: Human immunodeficiency virus type 1 (WMJ2 ISOLATE)
Expression system: synthetic construct
UniProt:
  • Canonical: P05880 (Residues: 662-674; Coverage: 2%)
Gene name: env

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 157.3Å b: 44.58Å c: 85.4Å
α: 90° β: 113.8° γ: 90°
R-values:
R R work R free
0.164 0.162 0.207
Expression systems:
  • Escherichia coli BL21(DE3)
  • synthetic construct

Quick links

Citations

2 review citations

Viral Membrane Fusion and the Transmembrane Domain.
Barrett et al. (2020)
1 more

3 mentions without citation

The Atomic Structure of the HIV-1 gp41 Transmembrane Domain and Its Connection to the Immunogenic Membrane-proximal External Region.
Apellániz et al. (2015)
2 more