4wy4

X-ray diffraction
1.4Å resolution

Crystal structure of autophagic SNARE complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Vesicle-associated membrane protein 8 Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BV40 (Residues: 11-74; Coverage: 64%)
Gene name: VAMP8
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-17 Chain: B
Molecule details ›
Chain: B
Length: 58 amino acids
Theoretical weight: 6.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56962 (Residues: 170-227; Coverage: 19%)
Gene name: STX17
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 29 Chain: C
Molecule details ›
Chain: C
Length: 78 amino acids
Theoretical weight: 8.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95721 (Residues: 39-116; Coverage: 30%)
Gene name: SNAP29
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 29 Chain: D
Molecule details ›
Chain: D
Length: 65 amino acids
Theoretical weight: 7.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95721 (Residues: 194-258; Coverage: 25%)
Gene name: SNAP29
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 82.85Å b: 35.03Å c: 104.2Å
α: 90° β: 94.09° γ: 90°
R-values:
R R work R free
0.138 0.136 0.177
Expression system: Escherichia coli BL21(DE3)