4wxk

X-ray diffraction
2.05Å resolution

Crystal structure of a peptide deformylase from Haemophilus influenzae

Released:
Entry authors: Kishor C, Addlagatta A

Function and Biology Details

Reaction catalysed:
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide deformylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 169 amino acids
Theoretical weight: 19.08 KDa
Source organism: Haemophilus influenzae 86-028NP
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4QMV6 (Residues: 1-169; Coverage: 100%)
Gene names: NTHI0725, def
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 41.036Å b: 80.961Å c: 98.586Å
α: 90° β: 97.81° γ: 90°
R-values:
R R work R free
0.164 0.161 0.222
Expression system: Escherichia coli BL21(DE3)