4wvh

X-ray diffraction
2.1Å resolution

Crystal structure of the Type-I signal peptidase from Staphylococcus aureus (SpsB) in complex with a substrate peptide (pep1).

Released:

Function and Biology Details

Reaction catalysed:
Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 533 amino acids
Theoretical weight: 59.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0AEX9 (Residues: 33-392; Coverage: 97%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
substrate peptide (pep1) Chain: C
Molecule details ›
Chain: C
Length: 6 amino acids
Theoretical weight: 667 Da
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 63.661Å b: 80.193Å c: 119.426Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.248
Expression systems:
  • Escherichia coli K-12
  • Not provided