X-ray diffraction
1.8Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GTP-binding nuclear protein Ran Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Ran-specific GTPase-activating protein 1 Chain: B
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 16.32 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
  • Canonical: P41920 (Residues: 62-201; Coverage: 70%)
Gene names: CST20, HTN1, SFO1, YD8119.08, YDR002W, YRB1
Sequence domains: RanBP1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Crm1p Chain: C
Molecule details ›
Chain: C
Length: 1024 amino acids
Theoretical weight: 117.47 KDa
Source organism: Saccharomyces cerevisiae R008
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 105.909Å b: 105.909Å c: 305.526Å
α: 90° β: 90° γ: 90°
R R work R free
0.139 0.136 0.184
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli