› 4wv3

X-ray diffraction
2.6Å resolution

Crystal structure of the anthranilate CoA ligase AuaEII in complex with anthranoyl-AMP

Released:
DOI: 10.2210/pdb4wv3/pdb
PDB 4wv3 coloured by chain and viewed from the front.
PDB 4wv3 coloured by chain and viewed from the side.
PDB 4wv3 coloured by chain and viewed from the top.
Source organism: Stigmatella aurantiaca
Primary publication:
Structural Insights into Anthranilate Priming during Type II Polyketide Biosynthesis.
Jackson DR, Tu SS, Nguyen M, Barajas JF, Schaub AJ, Krug D, Pistorius D, Luo R, M��ller R, Tsai SC
ACS Chem. Biol. (2015)
PMID: 26473393

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate-CoA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 529 amino acids
Theoretical weight: 58.68 KDa
Source organism: Stigmatella aurantiaca
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F3Y661 (Residues: 1-516; Coverage: 100%)
Gene name: auaEII
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 3UK 2 x 3UK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 50.054Å b: 132.219Å c: 160.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways.
Sulpizio et al. (2021)
2 more

1 mention without citation

Structural Insights into Anthranilate Priming during Type II Polyketide Biosynthesis.
Jackson et al. (2016)