X-ray diffraction
1.9Å resolution

N-terminal 43 kDa fragment of the E. coli DNA gyrase B subunit grown from 100 mM NaCl condition

Source organism: Escherichia coli K-12
Primary publication:
The role of monovalent cations in the ATPase reaction of DNA gyrase.
Acta Crystallogr. D Biol. Crystallogr. 71 996-1005 (2015)
PMID: 25849408

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA gyrase subunit B Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0AES6 (Residues: 2-393; Coverage: 49%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 87.91Å b: 140.88Å c: 79.52Å
α: 90° β: 90° γ: 90°
R R work R free
0.185 0.184 0.22
Expression system: Escherichia coli BL21(DE3)