4wub

X-ray diffraction
1.75Å resolution

N-terminal 43 kDa fragment of the E. coli DNA gyrase B subunit grown from 100 mM KCl condition

Released:
Source organism: Escherichia coli K-12
Entry authors: Hearnshaw SJ, Chung TT, Stevenson CEM, Maxwell A, Lawson DM

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AES6 (Residues: 2-393; Coverage: 49%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 87.88Å b: 140.9Å c: 79.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.204
Expression system: Escherichia coli BL21(DE3)