› 4wu2

X-ray diffraction
2.15Å resolution

Structure of the PTP-like myo-inositol phosphatase from Selenomonas ruminantium in complex with myo-inositol-(1,4,5)-trikisphosphate

Released:
DOI: 10.2210/pdb4wu2/pdb
PDB 4wu2 coloured by chain and viewed from the front.
PDB 4wu2 coloured by chain and viewed from the side.
PDB 4wu2 coloured by chain and viewed from the top.
Source organism: Selenomonas ruminantium
Primary publication:
Structure of the PTP-like phytase from Selenomonas ruminantium in complex with myo-inositol-(1,4,5)-trikisphosphate
Bruder LM
To be published

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine specific protein phosphatases domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 39.13 KDa
Source organism: Selenomonas ruminantium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WUJ1 (Residues: 28-346; Coverage: 100%)
Gene name: phyA
Sequence domains: Inositol hexakisphosphate
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand I3P 2 x I3P
Ligand PO4 2 x PO4
Ligand GOL 4 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 45.98Å b: 137.67Å c: 80.01Å
α: 90° β: 102.42° γ: 90°
R-values:
R R work R free
0.198 0.197 0.219
Expression system: Escherichia coli BL21(DE3)

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