› 4wt7

X-ray diffraction
2Å resolution

Crystal structure of an ABC transporter solute binding protein (IPR025997) from Agrobacterium vitis (Avi_5165, Target EFI-511223) with bound allitol

Released:
DOI: 10.2210/pdb4wt7/pdb
PDB 4wt7 coloured by chain and viewed from the front.
PDB 4wt7 coloured by chain and viewed from the side.
PDB 4wt7 coloured by chain and viewed from the top.
Source organism: Agrobacterium vitis S4
Entry authors: Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 34.49 KDa
Source organism: Agrobacterium vitis S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9K0E0 (Residues: 40-337; Coverage: 100%)
Gene names: Avi_5165, rbsB
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand X9X 2 x X9X
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 77.08Å b: 83.93Å c: 189.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.23
Expression system: Escherichia coli BL21(DE3)

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