X-ray diffraction
1.62Å resolution

Structure of the toluene 4-monooxygenase NADH oxidoreductase T4moF, K270S K271S variant


Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 35.89 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
  • Canonical: Q03304 (Residues: 2-326; Coverage: 100%)
Gene name: tmoF
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 84.334Å b: 69.074Å c: 68.224Å
α: 90° β: 116.53° γ: 90°
R R work R free
0.147 0.145 0.189
Expression system: Escherichia coli BL21