› 4wqd

X-ray diffraction
1.22Å resolution

Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum - K208G mutant

Released:
DOI: 10.2210/pdb4wqd/pdb
PDB 4wqd coloured by chain and viewed from the front.
PDB 4wqd coloured by chain and viewed from the side.
PDB 4wqd coloured by chain and viewed from the top.
Source organism: Allochromatium vinosum DSM 180
Primary publication:
Thiosulfate Dehydrogenase (TsdA) from Allochromatium vinosum: Structural and Functional Insights into Thiosulfate Oxidation.
Brito JA, Denkmann K, Pereira IA, Archer M, Dahl C
J. Biol. Chem. (2015)
PMID: 25673691

Function and Biology Details

Reaction catalysed: 
2 thiosulfate + 2 ferricytochrome c = tetrathionate + 2 ferrocytochrome c
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 26.87 KDa
Source organism: Allochromatium vinosum DSM 180
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3RVD4 (Residues: 29-270; Coverage: 100%)
Gene names: Alvin_0091, tsdA
Sequence domains: Cytochrome C oxidase, cbb3-type, subunit III
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEC 2 x HEC
4 bound ligands:
Ligand IOD 1 x IOD
Ligand EDO 1 x EDO
Ligand GAI 1 x GAI
Ligand SO4 1 x SO4
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 79.093Å b: 70.811Å c: 57.873Å
α: 90° β: 128.99° γ: 90°
R-values:
R R work R free
0.153 0.151 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Generation and Physiology of Hydrogen Sulfide and Reactive Sulfur Species in Bacteria.
Han et al. (2022)
1 more

1 mention without citation

Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum: structural and functional insights into thiosulfate oxidation.
Brito et al. (2015)