4wpl

X-ray diffraction
1.15Å resolution

Crystal structure of Mycobacterium tuberculosis uracil-DNA glycosylase in complex with uracil, Form I

Released:
Entry authors: Arif SM, Geethanandan K, Mishra P, Surolia A, Varshney U, Vijayan M

Function and Biology Details

Reaction catalysed:
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 25.81 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFQ9 (Residues: 1-227; Coverage: 100%)
Gene names: MTCY349.11, Rv2976c, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 38.91Å b: 63.9Å c: 45.08Å
α: 90° β: 112.23° γ: 90°
R-values:
R R work R free
0.105 0.104 0.124
Expression system: Escherichia coli BL21(DE3)