› 4wpb

X-ray diffraction
3.11Å resolution

Vascular endothelial growth factor in complex with alpha/beta-VEGF-1

Released:
DOI: 10.2210/pdb4wpb/pdb
PDB entry 4wpb coloured by chain, front view.
PDB entry 4wpb coloured by chain, side view.
PDB entry 4wpb coloured by chain, top view.
Source organisms:
Primary publication:
Targeting diverse protein-protein interaction interfaces with α/β-peptides derived from the Z-domain scaffold.
Checco JW, Kreitler DF, Thomas NC, Belair DG, Rettko NJ, Murphy WL, Forest KT, Gellman SH
Proc Natl Acad Sci U S A 112 4552-7 (2015)
PMID: 25825775

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147439 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N-VEGF Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15692 (Residues: 214-315; Coverage: 26%)
Gene names: VEGF, VEGFA
Sequence domains: VEGF heparin-binding domain
Structure domains: Cystine-knot cytokines
alpha/beta-VEGF-1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 40 amino acids
Theoretical weight: 4.59 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand B3D 2 x B3D
Ligand AIB 4 x AIB

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 39.1Å b: 78.4Å c: 56.5Å
α: 90° β: 102.8° γ: 90°
R-values:
R R work R free
0.245 0.24 0.298
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

Systematic Targeting of Protein-Protein Interactions.
Modell et al. (2016)
11 more

5 mentions without citation

Proteomimetics as protein-inspired scaffolds with defined tertiary folding patterns.
Horne et al. (2020)
4 more