X-ray diffraction
1.6Å resolution

Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature

Source organism: Staphylococcus aureus
Primary publication:
Three-dimensional diffuse x-ray scattering from crystals of Staphylococcal nuclease.
Proc. Natl. Acad. Sci. U.S.A. 94 6180-4 (1997)
PMID: 9177191

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.84 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
  • Canonical: P00644 (Residues: 83-231; Coverage: 73%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P41
Unit cell:
a: 48.499Å b: 48.499Å c: 63.43Å
α: 90° β: 90° γ: 90°
R R work R free
0.181 0.18 0.204
Expression system: Escherichia coli