X-ray diffraction
1.99Å resolution

Crystal structure of human wild-type GAPDH at 1.99 angstroms resolution


Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D, E, F, G, O
Molecule details ›
Chains: A, B, C, D, E, F, G, O
Length: 335 amino acids
Theoretical weight: 36.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P04406 (Residues: 1-335; Coverage: 100%)
Gene names: CDABP0047, GAPD, GAPDH, OK/SW-cl.12
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NAD 6 x NAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 132.537Å b: 81.423Å c: 147.802Å
α: 90° β: 90.06° γ: 90°
R R work R free
0.139 0.139 0.179
Expression system: Escherichia coli BL21(DE3)