4wlp

X-ray diffraction
3.1Å resolution

Crystal structure of UCH37-NFRKB Inhibited Deubiquitylating Complex

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5K5 (Residues: 5-322; Coverage: 96%)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains:
Nuclear factor related to kappa-B-binding protein Chain: B
Molecule details ›
Chain: B
Length: 114 amino acids
Theoretical weight: 13.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P4R8 (Residues: 40-153; Coverage: 9%)
Gene names: INO80G, NFRKB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P4122
Unit cell:
a: 95.488Å b: 95.488Å c: 131.964Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.193 0.268
Expression system: Escherichia coli