4wkt

X-ray diffraction
1.78Å resolution

n-Alkylboronic Acid Inhibitors Reveal Determinants of Ligand Specificity in the Quorum-Quenching and Siderophore Biosynthetic Enzyme PvdQ

Released:

Function and Biology Details

Reaction catalysed:
An N-acyl-L-homoserine lactone + H(2)O = L-homoserine lactone + a carboxylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acyl-homoserine lactone acylase PvdQ subunit beta Chain: C
Molecule details ›
Chain: C
Length: 548 amino acids
Theoretical weight: 60.63 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I194 (Residues: 217-762; Coverage: 74%)
Gene names: PA2385, pvdQ, qsc112
Sequence domains: Penicillin amidase
Structure domains:
Acyl-homoserine lactone acylase PvdQ subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 17.99 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I194 (Residues: 28-192; Coverage: 22%)
Gene names: PA2385, pvdQ, qsc112
Sequence domains: Penicillin amidase
Structure domains: Penicillin Amidohydrolase, domain 1

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 121.294Å b: 167.512Å c: 95.025Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.192
Expression system: Escherichia coli