X-ray diffraction
1.9Å resolution

Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with hydroxybutylthio-DADMe-Immucillin-A

Source organism: Helicobacter pylori J99
Primary publication:
New Antibiotic Candidates against Helicobacter pylori.
J. Am. Chem. Soc. 137 14275-80 (2015)
PMID: 26494017

Function and Biology Details

Reactions catalysed:
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Aminodeoxyfutalosine nucleosidase Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 26.89 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9ZMY2 (Residues: 2-230; Coverage: 100%)
Gene names: jhp_0082, mtn, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I432
Unit cell:
a: 157.256Å b: 157.256Å c: 157.256Å
α: 90° β: 90° γ: 90°
R R work R free
0.16 0.158 0.19
Expression system: Escherichia coli BL21(DE3)