4wkc

X-ray diffraction
1.64Å resolution

Crystal structure of Escherichia coli 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with butylthio-DADMe-Immucillin-A

Released:

Function and Biology Details

Reaction catalysed:
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 26.06 KDa
Source organism: Escherichia coli O139:H28 str. E24377A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7ZHQ1 (Residues: 1-232; Coverage: 100%)
Gene names: EcE24377A_0164, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 71.396Å b: 91.386Å c: 70.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.186
Expression system: Escherichia coli BL21(DE3)