X-ray diffraction
1.37Å resolution

Crystal structure of Vibrio cholerae 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with methylthio-DADMe-Immucillin-A


Function and Biology Details

Reaction catalysed:
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 26.23 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A5F5R2 (Residues: 1-231; Coverage: 100%)
Gene names: VC0395_A1957, VC395_2494, mtnN, pfs
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 53.192Å b: 72.002Å c: 61.648Å
α: 90° β: 110.32° γ: 90°
R R work R free
0.165 0.164 0.183
Expression system: Escherichia coli BL21(DE3)