› 4wk6

X-ray diffraction
2.21Å resolution

Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) (G141A) from Vibrio cholerae in complex with NADPH

Released:
DOI: 10.2210/pdb4wk6/pdb
PDB 4wk6 coloured by chain and viewed from the front.
PDB 4wk6 coloured by chain and viewed from the side.
PDB 4wk6 coloured by chain and viewed from the top.
Source organism: Vibrio cholerae 12129(1)
Primary publication:
Dissecting the structural elements for the activation of ��-ketoacyl-(acyl carrier protein) reductase from Vibrio cholerae.
Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W
J. Bacteriol. (2015)
PMID: 26553852

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier protein] reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 251 amino acids
Theoretical weight: 26.37 KDa
Source organism: Vibrio cholerae 12129(1)
Expression system: Escherichia coli
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NDP 4 x NDP
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 79.904Å b: 110.337Å c: 117.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.241
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Indole Biodegradation in Acinetobacter sp. Strain O153: Genetic and Biochemical Characterization.
Sadauskas et al. (2017)
5 more

1 mention without citation

Dissecting the Structural Elements for the Activation of β-Ketoacyl-(Acyl Carrier Protein) Reductase from Vibrio cholerae.
Hou et al. (2016)