› 4wk1

X-ray diffraction
1.98Å resolution

Crystal structure of Staphylococcus aureus PstA in complex with c-di-AMP

Released:
DOI: 10.2210/pdb4wk1/pdb
PDB 4wk1 coloured by chain and viewed from the front.
PDB 4wk1 coloured by chain and viewed from the side.
PDB 4wk1 coloured by chain and viewed from the top.
Source organism: Staphylococcus aureus subsp. aureus COL
Primary publication:
c-di-AMP recognition by Staphylococcus aureus PstA.
Müller M, Hopfner KP, Witte G
FEBS Lett. (2014)
PMID: 25435171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PstA Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2WXZ7 (Residues: 1-109; Coverage: 100%)
Gene name: SACOL0525
Sequence domains: Cyclic-di-AMP receptor
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand 2BA 1 x 2BA
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 68.1Å b: 68.1Å c: 135.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.188 0.229
Expression system: Escherichia coli BL21(DE3)

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Citations

10 review citations

Cyclic di-AMP, a second messenger of primary importance: tertiary structures and binding mechanisms.
He et al. (2020)
9 more